Laboratory and Clinical Medicine. Pharmacy

Scientific and practical quarterly peer-reviewed journal

 

ISSN 2712-9330 (Online)

VOL 4, No. 3 (2024)

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    Editorial Article

  • AUTHOR'S ACTIVITY OF JOURNAL LABORATORY AND CLINICAL MEDICINE. PHARMACIA. 2021–2022 OUTCOMES THROUGH THE PRISM OF COMPETITIVENESS

    • Pages: 4-10
    • Views: 52
    Abstract. According to elibrary3 reports, scientific and practical medical journal Laboratory and Clinical Medicine. Pharmacy (LCMP)4 which has been published 3 years takes well-deserved place along with well-known, authoritative and long-published scientific medical mass media. Increasing in twice the authors number (20 vs 39) and in 1.5 times new once (20 vs 29), upward trend of h-index (Hirsch index) (4.2 vs 4.9) and Gini index (0.55 vs 0.8), a noticeable drop of HHI (the Herfindahl–Hirschman index) for authors' organizations (1837 vs 1378), increase in 6-times the number of views per year (100 vs 625) and almost in 4 times the downloads of articles per year (38 vs 146), the two-year IF (Impact Factor), taking into account citation of all sources in Russian Science Citation Index (RSCI) – 0.692, as well as the possibility of publication in three branches of science, characterize the journal as promising and competitive in its industry, with a wide-spread geography of authors and medical organizations. In order to increase the citation of the LCMP, the main concept of the journal is to improve a manuscript. The results of the three-years LCMP work showed that the preparation of manuscripts according to the Author Guidelines should belong to editorial and publishing team. In order to avoid the loss of publication activity the editorial team of LCMP has all the necessary competencies for supporting and providing an author's manuscript.
  • Original Articles

  • COMPARATIVE ANALYSIS OF MICROBIOTA OF PROSTATIC SECRETION IN PATIENTS WITH CHRONIC BACTERIAL AND ABACTERIAL PROSTATITIS OF CATEGORY IIIA BY MASS SPECTROMETRY EXAMINATION OF MICROBIAL MARKERS

    • Pages: 11-23
    • Views: 59
    Introduction: Numerous studies have proven that Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is a common disease condition that has been the focus of attention in the global medical community due to its prevalence and the unsatisfactory treatment outcomes.
    The purpose of this study is to compare the microbiota of prostate secretions (PSM) between patients with chronic bacterial prostatitis (CBP) and chronic abacterial prostatitis (CAP) of category IIIa.
    Material and methods: A prospective comparative study was conducted at the SM Clinic Medical Center in Moscow from 2018 to 2023. 159 male patients with prostatitis-like symptoms aged 25 to 50 were selected. Patients were divided into two groups: group 1 included patients with CBP (n = 38) and group 2 included patients with category IIIa CAP with clinical manifestations of chronic prostatitis (n = 121). The study of PSM microbiota was performed using the method of nucleic acid amplification (MANC) with real-time polymerase chain reaction (PCR), using the Androflor test (MANC-PCR-RT/Androflor), and gas chromatography-mass spectrometry using the method of microbial mass spectrometry (MSMM).
    Results: A comparative analysis of the absolute indices of PSM microbiota based on MSMM data revealed that patients with CBP had a higher level of coccal and bacillary microbiota, as well as enterobacteria (of transient flora), along with the low level of anaerobic and resident microorganisms compared to a great number of patients with CAP stage IIIa (p < 0.05).
    A comparative analysis of relative indices of PSM microbiota based on MSMM data showed that patients with CBP had higher levels of coccal microbiota and gram (–) bacillus (of transient flora and normally not found in healthy individuals), as well as significantly lower levels (p < 0.05) of anaerobic and resident microorganism compared to patients in the CAP category IIIa group.
    Conclusion: The study found that patients with CAP had a higher microbial load of anaerobic and resident microorganisms. The patients with CBP had an increase in the levels of the most pathogenic forms of microorganisms, including transient and enterobacterial species.
  • Clinical Case

  • BASIC AND EXTENDED HUMAN SEMEN EXAMINATION IN INFERTILE MALE: SPERM SEDIMENT CYTOLOGY, SPERM DNA DAMAGE OF SPERMATOZOA, REACTIVE OXYGEN SPECIES IN NORMOZOOSPERMIA AND VISCOSIPATHY. CLINICAL REPORTS

    • Pages: 24-30
    • Views: 68
    Abstract. Currently, the diagnosis ‘normozoospermia’ independently and in combination with ‘viscosipathy’ does not exclude male infertility and stopping further examination of patient. Moreover, these semiological diagnoses seem to be the most important diagnostic criteria for determining the cause of a significant number of idiopathic infertility in couples who had unsuccessful attempt to conceive from 12 months or more. These clinical cases demonstrate the importance of combine approach to basic semen examination made it much more extended by ancillary technique e.g. sperm sediment cytology (SSC), fragmentation of sperm DNA (DFS) and of level of reactive oxygen species (ROS) estimation.
  • Abstract Reviews

  • THE ROLE OF MACROAUTOPHAGY IN THE PATHOGENESIS OF ENDOMETRIOSIS

    • Pages: 31-39
    • Views: 46
    Abstract: Endometriosis is a common gynecological condition characterized by the presence of endometrial-like tissue outside the uterus, causing chronic pelvic pain, dysmenorrhea, and infertility. Recent studies highlight the significant role of macroautophagy in the pathogenesis of endometriosis, although the mechanisms underlying the changes in basal autophagy levels in endometriotic lesions remain insufficiently understood. Macroautophagy is a vital cellular process for renewing and recycling damaged organelles and proteins, involving key structures like autophagosomes and lysosomes.
    Research indicates that autophagy levels in the endometrium vary depending on the menstrual cycle phase, with the most pronounced induction occurring during the secretory phase. In endometriosis, both eutopic endometrium and endometriotic lesions show reduced basal autophagy levels, though there are conflicting findings. The PI3K/Akt/mTOR signaling pathway, which is associated with autophagy, plays a crucial role in regulating cell proliferation in endometriosis. Additionally, autophagy influences apoptosis, cell adhesion, and interactions with estrogens, making it a key element in understanding the pathogenesis of endometriosis and a potential target for therapeutic interventions.
  • APPLICATION OF ARTIFICIAL INTELLIGENCE IN LYMPHOID TISSUE TUMOR DIAGNOSIS

    • Pages: 40-47
    • Views: 51
    Lesions of lymphoid and hematopoietic tissue are the leading cause of mortality in young population among all malignant entities. To support the patients it should provide timely a reliable verification, which is the task of pathology service. Along with increasing of morbidity and mortality from malignant entities also the burden on pathologists is increasing. High work-flow on physicians combined with lack staffing remain urgent problems in healthcare. Currently, the application of artificial intelligence (AI) to laboratory practice plays a crucial role in medicine. The application of software with generative pre-trained model can support a pathologist in the interpretation of histological pattern and diagnosis of lymphatic system tumor in both surgical and biopsy samples. Diagnostic decision support improves a quality and accuracy of diagnosis and optimize the work process. Abstract review provides an analysis of recent scientific studies investigating the potential use of AI in the diagnosis of malignant entities of lymphoid and hematopoietic tissue.
  • THE POSSIBILITIES OF CULTURAL AND NON-CULTURAL METHODS FOR DIAGNOSING THE TAXONOMIC STRUCTURE OF THE MICROBIOTA IN PATIENTS WITH CHRONIC ABACTERIAL PROSTATITIS

    • Pages: 48-57
    • Views: 47
    Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) remains a widespread disease with a wide range of clinical manifestations, as well as an unpredictable response to treatment. The etiology of CP/CPPS is probably multifactorial,
    and confirmation of the role of infection in its development remains a difficult and debatable problem. Application of novel research methods, including mass spectrometry of microbial markers makes it possible to identify and quantify bacteria of various groups, including anaerobes, viruses, fungi, and minimize sampling requirements. The presence of certain microorganisms and an increased total number of them in patients with CP/CPPS, compared with healthy people, indicates a possible association of infection with prostatitis and requires further study of this problem in order to obtain more reliable results.