VOL 6, No. 1 (2026)

An Interview with the Developer of the Professional Trichology Program Time to Grow, Maria Vladimirovna Khaldina

The work is devoted to the study of modern problems in the appointment of choleretic drugs by doctors and dispensation in pharmacy organizations against the background of the growing popularity of self-medication among the population. The results of a survey of pharmaceutical and medical workers, as well as pharmacy visitors, on over-the-counter choleretic drugs are presented. The data was collected through a voluntary anonymous survey of respondents. This sociological survey is part of the pre-project research for the development of a pharmaceutical information algorithm for over-the-counter administration of choleretic drugs.

The lecture addresses issues related to the cytological diagnosis of pathological changes in the glandular epithelium of the uterine cervix. The cytological criteria for the categories AGC-NOS (atypical glandular cells, not otherwise specified), AGC-FN (atypical glandular cells, favor neoplasia), AIS (adenocarcinoma in situ), and invasive adenocarcinoma of the endocervix and endometrium are described. For each category, key cytological characteristics are described, including group architecture, nuclear features, cytoplasmic features, and background characteristics. The diagnosis of glandular epithelial lesions remains a challenging task requiring a high level of cytological expertise. Improvement in diagnostic accuracy can be achieved through the use of liquid-based cytology, adherence to the standardized Bethesda System, continuous professional development, and the implementation of new technologies.

Background. Chimeric Antigen Receptor T-cell (CAR-T) therapy demonstrates significant potential in oncology; however, its application in solid tumors, particularly ovarian cancer, is subject to several limitations. The development of novel T-lymphocyte vectors specific to tumor antigens necessitates a comprehensive preclinical assessment, including the study of the pharmacokinetic profile. Objective. To establish the key pharmacokinetic parameters of the developed CAR-T cell product (GM-TLP) designed for the therapy of CA125-expressing tumors. Materials and methods. The study was conducted on outbred mice and Chinchilla rabbits. The animals received a single intravenous injection of genetically modified T-lymphocytes, transfected with the CA125-specific plasmid, at a dose of $10^6$ cells per injection. Pharmacokinetic parameters were assessed by the quantitative determination of plasmid DNA in the mononuclear cell fraction of the blood and homogenates of internal organs using real-time PCR (qPCR) over a period of 30 days post-administration. Results. It was found that the maximum concentration of plasmid DNA in the blood of animals is reached 30 minutes after injection. A rapid decrease in concentration was shown during the first day, followed by reaching a plateau (11.2 – 13 % of cmax in the period from 3 to 12 days. By the 30th day, the concentration was approaching the background values. Rapid distribution of GM-TLP into parenchymal organs was noted: maximum concentrations in the spleen (44.8 ± 8.98 pg/ml), liver (25.4 ± 4.56 pg/ml), lungs (18.9 ± 3.87 pg/ml) and thymus (12.3 ± 2.54 pg/ml) were also recorded after 30 minutes. The modified lymphocytes and plasmid DNA persisted in the studied tissues during the entire follow-up period (30 days). Conclusion. The obtained pharmacokinetic data confirm the ability of the developed CAR-T cell product for rapid distribution and sustained persistence in the body of experimental animals. This prolonged persistence is a critically important factor for ensuring a sustained antitumor effect. The established pharmacokinetic parameters provide a solid basis for the further development and optimization of the therapeutic strategy.

Loose Anagen Hair Syndrome (LAHS) is a rare form of non-scarring alopecia characterized by disruption of hair shaft anchorage in the follicle during the anagen phase. The disease is most often diagnosed in children, primarily girls. This article presents current data on the pathogenesis and diagnosis of the syndrome and describes a clinical case.

Azo compounds, or aniline dyes, have a broad spectrum of antimicrobial activity. This has led to their widespread use in agriculture to protect crops, as well as in industrial processes and technologies to protect various materials (plastics, wood, rubber, etc.) from microbial attack. At the same time, the use of azo dyes in medicine is limited by their high toxicity and potential carcinogenicity. Resistance to azo dyes develops extremely slowly in clinical strains of bacteria and fungi due to their limited exposure to these compounds. Consequently, the sensitivity values of microbial cultures to azo dyes will remain stable over a long period of time. It has been established that strains of the same species are characterized by marked heterogeneity in their sensitivity to azo dyes. These stable characteristics of bacterial and fungal strains are required for conducting epidemiological analysis, using them as phenotypic epidemiological markers. Repeated isolation of cultures with similar indicators from objects in the nosocomial environment can be regarded as evidence of microorganisms’ circulation in the nosocomial environment, which is considered one of the main signs of nosocomial (hospital) strains.