Introduction: Numerous studies have proven that Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is a common disease condition that has been the focus of attention in the global medical community due to its prevalence and the unsatisfactory treatment outcomes.
The purpose of this study is to compare the microbiota of prostate secretions (PSM) between patients with chronic bacterial prostatitis (CBP) and chronic abacterial prostatitis (CAP) of category IIIa.
Material and methods: A prospective comparative study was conducted at the SM Clinic Medical Center in Moscow from 2018 to 2023. 159 male patients with prostatitis-like symptoms aged 25 to 50 were selected. Patients were divided into two groups: group 1 included patients with CBP (n = 38) and group 2 included patients with category IIIa CAP with clinical manifestations of chronic prostatitis (n = 121). The study of PSM microbiota was performed using the method of nucleic acid amplification (MANC) with real-time polymerase chain reaction (PCR), using the Androflor test (MANC-PCR-RT/Androflor), and gas chromatography-mass spectrometry using the method of microbial mass spectrometry (MSMM).
Results: A comparative analysis of the absolute indices of PSM microbiota based on MSMM data revealed that patients with CBP had a higher level of coccal and bacillary microbiota, as well as enterobacteria (of transient flora), along with the low level of anaerobic and resident microorganisms compared to a great number of patients with CAP stage IIIa (p < 0.05).
A comparative analysis of relative indices of PSM microbiota based on MSMM data showed that patients with CBP had higher levels of coccal microbiota and gram (–) bacillus (of transient flora and normally not found in healthy individuals), as well as significantly lower levels (p < 0.05) of anaerobic and resident microorganism compared to patients in the CAP category IIIa group.
Conclusion: The study found that patients with CAP had a higher microbial load of anaerobic and resident microorganisms. The patients with CBP had an increase in the levels of the most pathogenic forms of microorganisms, including transient and enterobacterial species.
Zieratsho A. Kadyrov – MD, professor, the head of the department of endoscopic urology and ultrasound diagnostic of faculty of continuous medical education of medical institute in ‘Peoples’ Friendship University of Russia’, Moscow, Russia. E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.; This email address is being protected from spambots. You need JavaScript enabled to view it.
Shota V. Ramishvili – postgraduate student of the department of endoscopic urology and ultrasound diagnostic of faculty of continuous medical education of medical institute in ‘Peoples’ Friendship University of Russia’, Moscow, Russia. E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Vladimir S. Stepanov– assistant of endoscopic urology and ultrasound guidance department of faculty of postgraduate education of faculty of medical institute in ‘Peoples’ Friendship University of Russia’, Moscow, Russia. E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Aleksei V. Ignatev – postgraduate student of the department of endoscopic urology and ultrasound diagnostic of faculty of continuous medical education of medical institute in ‘Peoples’ Friendship University of Russia’, Moscow, Russia. E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Shakro G. Mashaneishvili – postgraduate student of the department of endoscopic urology and ultrasound diagnostic of faculty of continuous medical education of medical institute in ‘Peoples’ Friendship University of Russia’, Moscow, Russia. E-mail: -
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