Aim. To analyze the relationship between semiological diagnosis and SSC (sperm sediment cytology)-bacteriospermia (SSC-mB “+”) in infertile male.
Results. 162 men (162; 30.62 %) with various combinations of semiological diagnoses were presented by SSC-mB “+”. According to investigation, SSC-mB “+” has a relationship with pathospermia. Mostly, the combinations of semiological diagnoses like agglutination, dyskinesia, asthenozoospermia, necrozoospermia, oligostenozoteratozoospermia and viscosipathy are attended SSC-mB “+”. The combinations of semiological diagnoses like pyospermia, oligoastenozoospermia, hemospermia less often are atttended SSC-mB “+”. Semiological diagnoses of oligospermia, azooospermia, cryptozoospermia and normozoospermia most are not attended SSC-mB “+”. If oligoastenotheratozoospermia, oligoastenozoospermia, viscosipathy, pyospermia, and agglutination are presented there is a high probability of SSC-mB “+”. If necrozoospermia is presented there is a low probability of SSC-mB “+”. If asthenozoospermia, dyskinesia, cryptozoospermia, azoospermia, hemospermia, and oligospermia are presented the assumption of SSC-mB “+” is unlikely.
Conclusion. The data can provide the attending physician with evidence-based information about the relationship between SSC-mB “+” and semiological diagnoses that will support a correct interpretation the outcomes of easy-to-perform microscopic methods “Basic semen examination+SSC”-ROSE which can claim to be screening for detection of bacteriospermia.
Zhanna Yu. Davidova – MD, PhD, The International Cytology School & Innovatory Medical School, Head, Moscow, Russia; Medical Laboratory, Podolsk Diagnostic Center, Head, Podolsk, Russia. E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
1. World Health Organization. Sexually transmitted infections (STIs) Geneva: World Health Organization; 2019. https://www.who.int/news-room/fact-sheets/detail/sexually-transmitted-infections-(stis) [Google Scholar].
2. Ye H, Song T, Zeng X, et al. Association between genital mycoplasmas infection and human papillomavirus infection, abnormal cervical cytopathology, and cervical cancer: a systematic review and meta-analysis. Arch Gynecol Obstet. 2018Jun;297(6):1377-87.
3. Global Health Sector Strategy on Sexually Transmitted Infections. 2016 – 2021. World Health Organization, 2016. Available from: http://www.who.int/mediacentre/factsheets/fs110/ru/
4. Khan J, Farzand R. Prevalence of Mycoplasma hominis and Ureaplasma urealyticum among women with unexplaind infertility with and without vaginitis and cervicitis. African Journal of Microbiology Research. 2011;5(8):861-4.
5. Krasnosel'skih TV, Manasheva EB, Gezej MA. Komorbidnost' sifilisa i VICH-infekcii: otricatel'nyj epidemiologicheskij i klinicheskij sinergizm. VICH-infekciya i immunosupressii. 2018;10(3):7-16 (In Russ).
6. Klinicheskie rekomendacii po vedeniyu bol'nyh infekciyami, peredavaemymi polovym putem, i urogenital'nymi infekciyami. M.: DEKS-press, 2012. 112 р. (In Russ).
7. Alyaev YuG, Glybochko PV, Pushkar DYu, editors. Urology. Russian clinical guidelines. Moscow: GEOTAR-Media; 2016. 496 р. (In Russ).
8. EAU Guidelines. Edn. presented at the EAU Annual Congress, Amsterdam, the Netherlands, 2020. ISBN 978-94-92671-07-3.
9. Ahmadi MH, Mirsalehian A, Gilani MA Sadighi, et al. Association of asymptomatic Chlamydia trachomatis infection with male infertility and the effect of antibiotic therapy in improvement of semen quality in infected infertile men. Andrologia. 2018;50:e12944. DOI: 10.1111/and.12944
10. Yang Y, Jia CW, Ma YM, et al. Correlation between HPV sperm infection and male infertility. Asian J Androl. 2013;15:529-32. PMID: 23603919. DOI: 10.1038/ aja.2013.36
11. Damke E, Kurscheidt FA, Balani VA, et al. Male Partners of Infertile Couples with Seminal Infections of Human Papil- lomavirus Have Impaired Fertility Parameters. Biomed Res Int. 2017:4684629. PMID: 28835893. DOI: 10.1155/2017/4684629
12. Boeri L, Capogrosso P, Ventimiglia E, et al. High-risk human papillomavirus in semen is associated with poor sperm progressive motility and a high sperm DNA fragmentation index in infertile men. Hum Reprod. 2019;34:209-17.
13. Ricci S, De Giorgi S, Lazzeri E, et al. Impact of asymptomatic genital tract infections on in vitro fertilization (IVF) outcome. PLoS One. 2018;13(11):e0207684. https://doi.org/10.1371/journal. pone. 0207684
14. Lin HP, Lu HX. Analysis of detection and antimicrobial resistance of pathogens in prostatic secretion from 1186 infertile men with chronic prostatitis. Zhonghua Nan Ke Xue. 2007Jul;13(7):628-31.
15. Boeri L, Pederzoli F, Capogrosso P, et al. Semen infections in men with primary infertility in the real-life setting. Fertil Steril. 2020Jun;113(6):1174-1182. DOI: 10.1016/j.fertnstert. 2020.01.034
16. Schuppe HC, Pilatz A, Hossain H, et al. Urogenital Infection as a Risk Factor for Male Infertility. Dtsch Arztebl Int. 2017May12;114(19):339-346. DOI: 10.3238/arztebl.2017.0339
17. Muzhskoe besplodie. Klinicheskie rekomendatsii. 2021. Available at: https://cr.minzdrav.gov.ru/schema/5_2 Accessed: 20 May 2023 (In Russ).
18. Benelli A, Hossain H, Pilatz AM, et al. Prostatitis and its Management. European Urology Supplements. 2017;16:132-7.
19. Voroshilina ES, Zornikov DL, Panacheva EA. Sravnitel'noe issledovanie mikrobioty eyakulyata metodom kolichestvennoj PCR i kul'tural'nym metodom. Vestnik RGMU. 2019;1:44-49. DOI: 10.24075/vrgmu. 2019.009
20. Pochernikov DG., Postovojtenko NT., Strel'nikov AI. Sravnitel'nyj analiz kul'tural'nogo i molekulyarno-geneticheskogo metodov v issledovanii mikrobioty eyakulyata pri muzhskoj infertil'nosti. Andrologiya i genital'naya hirurgiya. 2019;20(2):40-47. (in Russ) https://doi.org/10.17650/2070-9781-2019-20-2-40-47
21. Borovec SYu. Diagnosticheskaya znachimost' issledovaniya mikroflory eyakulyata u bol'nyh hronicheskim bakterial'nym prostatitom metodom PCR-RT ?Androflor?. Urologicheskie vedomosti. 2019;9(1S):22-23 (In Russ).
22. Sapozhkova Z, Kasoyan K, Kovalchuk E, et al. Sperm Sediment Cytology: A New Technique for Diagnosing Occult Urologic Infections. Acta Cytologica. 2017;61(3):247-251. DOI:10.1159/000469653
23. Sapozhkova ZY, Pochernikov DG, Galkina IS. Infectious and non-infectious findings in infertile men with asymptomatic chronic prostatitis: sperm sediment cytology vs DNA screening. Acta Cytologica. 2019;63(suppl 1):89.
24. Sapozhkova ZhYu, Nikanorova AV, Patsap OI, et al. The Role of Sperm Sediment Cytology in Non-Invasive Diagnostics of Seminoma. Case report. Laboratory and Clinical Medicine. Pharmacy. 2022;2(2):44-59 (In Russ).
25. Pochernikov DG, Sapozhkova ZhYu. PCR “checkup” in male in the sexually transmitted infections. Trend watching. Laboratory and Clinical Medicine. Pharmacy. 2022;2(3):18-26 (In Russ). DOI: 10.14489/lcmp. 2022.03.pp.018-026
26. Prikaz Minzdrava Rossii ot 18.05.2021 No 464n ?Ob utverzhdenii Pravil laboratornyh issledovanij?. https://base.garant.ru/400839855/ Accessed: 20.05.2023.
27. Sapozhkova ZhYu, Milovanova GA, Pochernikov DG, et al. Diagnostic capabilities of ejaculate sediment examination with the help of nucleic acid amplification by polymerase chain reaction and microbiologic examination in searching for causes of male reproductive disfunction. Medical Technologies. Assessment and Choice. 2022;44(2):62-71 (In Russ).
28. https://doi.org/10.17116/medtech20224402162 Accessed: 20.05.2023 (In Russ).
29. Sapozhkova ZhYu, Pochernikov DG, Galkina IS. Rol' tsitologii osadka eyakulyata i PTsR-diagnostiki v poiske vozmozhnoi prichiny muzhskogo besplodiya i rannei onkopatologii. Tezisy Pervogo natsional'nogo kongressa s mezhdunaronym uchastiem LABRiN. Nauchno-prakticheskii zhurnal ?Akusherstvo i ginekologiya?. Prilozhenie. 2019;4:75-76.
30. Sapozhkova ZhYu. Informativnost' kombinirovannogo analiza eyakulyata cheloveka v poiskah prichin narusheniya reproduktivnoj funkcii: spermogramma i citologicheskoe issledovanie osadka eyakulyata (COE). Laboratornaya i klinicheskaya medicina. Farmaciya. 2023;3(1):19-36. (In Russ). DOI: 10.14489/lcmp.2023.01.pp.019-036
31. Rebrova OYu. Statisticheskii analiz meditsinskikh dannykh. Primenenie paketa prikladnykh programm STATISTICA. Moscow: MediaSfera; 2002. 312 p.
32. Vspomogatel'nye reproduktivnye tekhnologii i iskusstvennaya inseminatsiya (protokol lecheniya). Klinicheskie rekomendatsii. Available at: https://rahr.ru/d_pech_mat_metod/ВРТ1.pdf. Accessed: 20 May 2023 (In Russ).
33. Zhenskoe besplodie. Klinicheskie rekomendatsii. 2021. Available at: https://rahr.ru/d_pech_mat_metod/ gbesp.pdf. Accessed: 20 May 2023 (In Russ).
34. WHO laboratory manual for the examination and processing of human semen. Sixth Edition. World Health Organization (2021) Available at: https://www.who.int/ publications/i/item/9789240030787. Accessed:20.05.2023.
35. Sapozhkova ZhYu, Shabalova IP, Kasoyan KT. Issledovanie osadka eyakulyata v diagnostike infektsii, peredavaemykh polovym putem: Uchebnoe posobie. Moscow: FGBOU DPO RMANPO MZ RF; 2017. P. 27 (In Russ).
36. Sapozhkova ZhYu. Patent na intellektual'nuyu sobstvennost' ?Sposob Laboratornoi Diagnostiki Muzhskoi Reproduktivnoi Funktsii?. Zayavka 2022113772/14 (028779). (In Russ).
37. Sapozhkova ZhYu. Patent na intellektual'nuyu sobstvennost' ?Sposob mikroskopicheskoi diagnostiki kachestva spermy posle sedimentatsii eyakulyata?, 2019. 686685 Available at: http://new.fips.ru. Accessed 20.05.2023 (In Russ).
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