ТЕХНОЛОГИЧЕСКОЕ РЕШЕНИЕ ДЛЯ ОЦЕНКИ АКТИВНЫХ ФОРМ КИСЛОРОДА И ДИСПЕРСИИ ХРОМАТИНА СПЕРМАТОЗОИДОВ: АДАПТАЦИЯ РЕКОМЕНДАЦИЙ ЛАБОРАТОРНОГО РУКОВОДСТВА ВОЗ (2021) К ПРАКТИКЕ МЕДИЦИНСКИХ ЛАБОРАТОРИЙ РОССИИ

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Авторы:

Давидова Ж. Ю.

- ТОМ 3, № 3 (2023)
DOI: 10.14489/lcmp.2023.03.pp.047-066
Тип статьи: Научная статья

Сквозной номер выпуска: 9
Страницы статьи: 47-66
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Рубрика: Новый взгляд из клинической и фармацевтической практики

Согласно лабораторному руководству Всемирной организации здравоохранения (ВОЗ) по исследованию и обработке спермы человека, 6-е издание (2021 г.) [1], существует ряд методов измерения уровней активных форм кислорода (АФК), фрагментации ДНК сперматозоидов (ДФС) и его индекса (DFI/DNA fragmentation index) в сперме, каждому из них присущи свои ограничения. Ввиду простоты исполнения не только в лабораторных условиях, но и местах оказания медицинской помощи (point-of-care/POC), мировым профессиональным сообществом отмечены два метода измерения АФК и ДФС как наиболее надежные: определение общей антиоксидантной способности спермы (ОС) и Гало-тест (Halo test/SCD /Sperm chromatin dispersion). Впервые в России были созданы два Набора реагентов (далее Набор): ?ОксиСперм (NBT-тест)? (Россия) по методике с нитросиним тетразолием (NBT-тест/Tetrazolium Nitroblue) для измерения АФК и Гало-тест (SCD) ?ГалоСперм? для оценки дисперсии хроматина сперматозоидов или ФДНКС. Оба Набора показали не только воспроизводимые результаты о сравнению с ВОЗ (2021), но и технологические преимущества по сравнению с описанными ВОЗ методами. В настоящей публикации представлены пошаговые инструкции по эксплуатации новых российских Наборов.

Жанна Юрьевна Давидова – к.м.н., руководитель Международной Школы Цитологии и Медицинской Школы Инноваций, Москва, Россия; руководитель клинико-диагностической лаборатории, Подольский диагностический центр, Подольск, Россия. E-mail: Адрес электронной почты защищен от спам-ботов. Для просмотра адреса в вашем браузере должен быть включен Javascript.

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DOI: 10.14489/lcmp.2023.03.pp.047-066
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Ключевые слова

мужское бесплодие, фрагментация ДНК, АФК, ДФС, NBT-тест, Гало-тест, ОксиСперм, Компания Лайф энд Кволити.

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Давидова Ж. Ю.Технологическое решение для оценки активных форм кислорода и дисперсии хроматина сперматозоидов: адаптация рекомендаций лабораторного руководства ВОЗ (2021) к практике медицинских лабораторий России // Лабораторная и клиническая медицина. Фармация. 2023. Т. 3, № 3. С. 47 – 66. DOI: 10.14489/ lcmp.2023.03.pp.047-066