Laboratory and Clinical Medicine. Pharmacy

Scientific and practical quarterly peer-reviewed journal

 

ISSN 2712-9330 (Online)

  • Continuous numbering: 14
  • Pages: 14-25
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Heading: Critical Review

Over the past two decades scientific and practical research and developments by Russian laboratory medicine specialists have been shown evolutionary and revolutionary achievements in the field of male infertility (MI) diagnostic technologies. Todays the current national guidelines contain information on laboratory research methods which should be reviewed and updated considering a key opinion leader of laboratory medicine. Review contains a detailed analysis of the clinical recommendations of the Ministry of Health of Russian Federation, the standard of medical care, GOST R ISO 23162–2023 and other normative legal documents which based on the algorithm of laboratory evaluation a cause of MI in ejaculate samples.
Zhanna Yu. Sapozhkova – MD, PhD, International Cytology School&Innovatory Medical School, Head, Senior Lecturer, Moscow, Russia. E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
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6. EAU Guidelines. Edn. presented at the EAU Annual Congress, Amsterdam, the Netherlands; 2020.
7. Sapozhkova ZhYu., Selivanov TO., Negasheva E.S., i dr. Interpretaciya laboratornyh issledovanij pri infekciyah muzhskih polovyh zhelez i narushenii reproduktivnoj funkcii. 2023
8. Davidova ZhYu. The Relationship between Semiological Diagnosis and SSC-bacteriospermia in Infertile Male. Laboratory and Clinical Medicine. Pharmacy. 2023;3(2):19-36. (In Russ). DOI: 10.14489/lcmp.2023.02.pp.019-036
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16. Pochernikov DG, Sapozhkova ZhYu. PCR ‘checkup’ in male in the sexually transmitted infections. Trend watching. Laboratory and Clinical Medicine. Pharmacy. 2022;2(3):18-26. (In Russ). DOI: 10.14489/lcmp.2022.03.pp.018-026
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20. Pochernikov DG, Vitvichkaya YuG, Boldyreva MN., et al. The information content of biological material for the study of the urogenital tract in men by real-time PCR (pilot trail). Experimental and Clinical Urology. 2019;(2):128-32.
21. Zhou YH, Ma HX, Yang Y, et al. Prevalence and anti-microbial resistance of Ureaplasma spp. and Mycoplasma hominis isolated from semen samples of infertile men in Shanghai, China from 2011 to 2016. European Journal of Clinical Microbiology & Infectious Diseases. 2018;37(4):729-34. DOI: 10.1007/s10096-017-3167-5
22. Pochernikov DG, Postovoitenko NT, Strelnikov AI. Comparative analysis of cultural and molecular genetic methods in the study of the microbiota of ejaculate in male infertility. Andrologiya i genital’naya hirurgiya. 2019;20(2):40-7. (In Russ). DOI: 10.17650/2070-9781-2019-20-2-40-47
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25. Pochernikov DG, Postovoitenko NT, Davidova ZhYu. The benefits from update laboratory preanalytical stage in PCR on the way to identify occult urological infections in male. Laboratory and Clinical Medicine. Pharmacy. 2023;3(2):4-11. (In Russ). DOI: 10.14489/lcmp.2023.02.pp.004-011
26. Sapozhkova ZhYu, Milovanova GA, Pochernikov DG, et al. Diagnostic capabilities of ejaculate sediment examination with the help of nucleic acid amplification by polymerase chain reaction and microbiologic examination in searching for causes of male reproductive disfunction. Medical Technologies. Assessment and Choice. 2022;44(2):62?71. (In Russ). DOI: 10.17116/medtech20224402162
27. Sapozhkova ZhYu, SHabalova IP., Kasoyan KT.: Uchebnoe posobie. Issledovanie osadka eyakulyata v diagnostike infekcij, peredavaemyh polovym putem. Moskva: FGBOU DPO RMANPO MZ RF; 2017. (In Russ).
28. Sapozhkova ZhYu, Kasoyan K., Kovalchuk E., et al. Sperm sediment cytology: a new technique for diagnosing occult urologic infections. Acta Cytologica. 2017;61:247-51. DOI: 10.1159/000469653b
29. Sapozhkova ZhYu, Kasoyan KT, SHabalova IP. Papillomavirusnaya infekciya u muzhchin: vozmozhnosti citologicheskogo issledovaniya osadka eyakulyata i VPCH-DNK testirovanie, klinicheskie nablyudeniya. Tezisy III Rossijskogo kongressa laboratornoj mediciny. Laboratornaya sluzhba. 2017;6(3):53-4. (In Russ).
30. Sapozhkova ZhYu, Makolin IA., SHatalov YuN. Voprosy diagnostiki urogenital’nyh infekcij u muzhchin. Aktual’nye voprosy medicinskoi? praktiki: sbornik nauchnyh trudov. Vypusk 2. Moskva: OAO ‘Medicina’; 2018 (In Russ).
31. Sapozhkova ZhYu, Pochernikov DG., Galkina IS. Rol’ citologii osadka eyakulyata i PCR-diagnostiki v poiske vozmozhnoj prichiny muzhskogo besplodiya i rannej onkopatologii. Tezisy Pervogo nacional’nogo kongressa s mezhdunarodnym uchastiem LABRiN. Nauchno-prakticheskij zhurnal ‘Akusherstvo i ginekologiya’. 2019;(Suppl 4):75-6. (In Russ).
32. Sapozhkova ZhYu. Sposob mikroskopicheskoj diagnostiki kachestva spermy posle sedimentacii eyakulyata. Patent RF 2 686. 19.11.2021. Byull. №32 (In Russ).
33. Sapozhkova ZhYu, Pochernikov DG, Galkina IS. Infectioous and non-infectious findings in infertile men with asymptomatic chronic prostatitis: Sperm Sediment Cytology vs DNA-screening 20th International Cytology Congress. 2019;96:89
34. Sapozhkova ZhYu. Informativeness of combined human semen analysis on the way to clarify the causes of reproductive dysfunction: basic semen examination and sperm sediment cytology (SSC). Laboratory and Clinical Medicine. Pharmacy. 2023;3(1):19-36. (In Russ). DOI: 10.14489/lcmp.2023.01.pp.019-036
35. Sapozhkova ZhYu. Sposob laboratornoj diagnostiki muzhskoj reproduktivnoj funkcii na baze ocenki dispersii DNK-fragmentov spermatozoidov. Patent RUS 2 795567. 05.05.2023. Byul. №13 (In Russ).
36. Davidova ZhYu, Chelikova GA. The role of sperm sediment cytology in diagnostics of high risk human papillomavirus. Laboratory and Clinical Medicine. Pharmacy. 2023;3(4):30-6. (In Russ). DOI: 10.14489/lcmp.2023.04.pp.030-036
37. Davidova ZhYu, Chelikova GA. Sperm sediment cytology on the way to male infertility check-up. Laboratory Service. 2023;12(4):51?4. (In Russ). DOI: 10.17116/labs20231204151
38. Davidova ZhYu, Chelikova GA. The role of multidisciplinary approach on the way to search an effective diagnostic algorithms in laboratory examination of patients with male accessory gland infections. Laboratory and Clinical Medicine. Pharmacy. 2024;4(1):17-24. (In Russ). DOI: 10.14489/lcmp.2024.01.pp.017-024
39. Pochernikov DG, Parshentsev AS, Filippova OS, et al. The role of three-stage examination on the way to searching a causes infertility in male. Clinical Report. Laboratory and Clinical Medicine. Pharmacy. 2024;4(1):34-42. (In Russ). DOI: 10.14489/lcmp.2024.01.pp.034-042
40. Davidova ZhYu. Sposob issledovaniya muzhskoj reproduktivnoj funkcii. Patent RUS № 2 819094. Published. 14.05.2024. Byul. №14 (In Russ).
41. Sapozhkova ZhYu. Basic and extended human semen examination in infertile male: sperm sediment cytology, sperm dna damage of spermatozoa, reactive oxygen species in normozoospermia and viscosipathy. Clinical Reports. Laboratory and Clinical Medicine. Pharmacy. 2024;4(3):24-30. (In Russ). DOI: 10.14489/lcmp.2024.03.pp.024-030
42. Sapozhkova ZhYu, Eremin KI, Patsap OI. Sperm Chromatin Dispersion Test for Examination of Infertile Male: From Сlinical Trials of First Russian Kit GEMSTANDART–HaloSperm L&Q. Laboratory and Clinical Medicine. Pharmacy. 2022;2(3):37-56. (In Russ). DOI: 10.14489/ lcmp.2022.03.pp.037-056
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49. Sapozhkova ZhYu, Eremin KI. Patent on Invention. Method for combined measurement of concentration of peroxidase-positive cells (neutrophilic granulocytes) and sperm cells in human ejaculate using variations based on cytochemical staining. 2020 г. © 2 726207. (In Russ).
50. Sapozhkova ZhYu, Milovanova GA, Patsap OI. Laboratory diagnostics of male infertility. Biomarkers and methods. Part II. Laboratory and Clinical Medicine. Pharmacy. 2021;1(2):65-79. (In Russ). DOI: 10.14489/lcmp.2021.02.pp.065-079
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55. Sapozhkova ZhYu, Eremin KI. Updates in protocol for human semen examination. Klinicheskaya Laboratornaya Diagnostica (Russian Clinical Laboratory Diagnostics). 2020;65(2):106-10 (In Russ.) DOI: 10.18821/0869-2084-2020-65-2-106-110
56. Sapozhkova ZhYu, Eremin KI, Dolgov VV. Unification of procedures of cytochemical staining of human ejaculate to determine the true fertility of men. Laboratory Diagnostics. Eastern Europe. 2020;9(1-2):41-9 (In Russ) DOI: 10.34883/PI.2020.9.1.026

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DOI: 10.14489/lcmp.2024.04.pp.014-025
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Sapozhkova ZhYu. Semen Examination on the National Way. Laboratory and Clinical Medicine. Pharmacy. 2024;4(4):14-25. (In Russ). DOI: 10.14489/lcmp.2024.04.pp.014-025