Relevance. During the regeneration of a skin wound there is a changing an inflammation phases and chemical content, as a result, a pH shift. Study of dependence the stage of the wound process from pH of the wound bed is increasable important for medication strategy by drags which changing pH and accelerate the healing of a skin wound.
Materials and methods. The experiment was performed by modeling a purulent wound in 36 animals – a Wistar rat. Further, on the 1st, 3rd, 5th, 8th, 10th and 15th days of the experiment, the pH of the wounds was determined and autopsy material was collected for subsequent histological examination. The obtained sections were stained with hematoxylin and eosin, a descriptive assessment of the wound defect was performed, as well as the calculation of cellular components.
Results. The wound bed was filled with purulent necrotic masses on the 3rd day of the experiment, while the cellular composition was mainly represented by granulocytes and macrophages. At the same time, the pH measurement has been determined the value of 7.64 (7.48; 7.81), cellular index (CI) 0.4 (0.36; 0.43). From the 8th day of the experiment a morphometric study has been showed the spontaneous healing of a purulent wound with changing an exudative phase to proliferative one. That point has been confirmed the CI 1.3 (1.25; 1.41) witch pH acidification to 7.39 (7.20;7.76).
Conclusion. According to the study, when the pH value decreases, it leads to an increase in the number of fibroblastic cells and has a beneficial effect on the course of the wound process.
Ekaterina S. Mishina – PhD, Assistant Professor Department of Histology, Embriology, Citology, Kursk State Medical University, Kursk, Russia. E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Arsen Yu. Grigoryan – PhD, Аssistant Рrofessor Department of Оperative Surgery and Topographic Anatomy, Kursk State Medical University, Kursk, Russia.E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Maria A. Zatolokina – Doctor of Medical Sciences, Professor, Professor of the Department of Histology, Embryology, Cytology of Kursk State Medical University, Kursk, Russia. E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Tatyana N. Kudryavtseva – PhD, Associate Professor, Head of the Research Laboratory of Organic Synthesis at Kursk State University, Kursk, Russia. E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Sergey A. Efanov – Senior Researcher, Head of the Research Laboratory of Organic Synthesis, Kursk State University, Kursk, Russia. E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Vitaly V. Soroka – Assistant at the Department of Chemistry at Kursk State University, Kursk, Russia. E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
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