COMPARATIVE ANALYSIS OF APOPTOSIS AND HYPOXIA IN PLACENTAS OF PATIENTS WITH COVID-19 AND CRITICAL LUNG LESION (IMMUNOHISTOCHEMICAL EXAMINATION)

Details: Created: 30 June 2024; Hits: 631

Autors:

Voropaeva E. E. , Hajdukova Y. V. , Holopova A. Y. , Alieva A. A. , Kazachkova E. A. , Kazachkov E. L.

- VOL 4, No. 2 (2024)
DOI: 10.14489/lcmp.2024.02.pp.025-034
Article type: Research Article

Continuous numbering: 12
Pages: 25-34
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Heading: Original Articles

Relevance. The risk of developing severe stage of COVID-19 infection in pregnant women is quite high. The study of apoptosis and hypoxia in placenta which have a fundamental impact on functioning of this organ in COVID-19 and lung lesion of varying severity is important for the justification of obstetric tactics. The aim of the study was to determine the expression level of apoptosis-inducing factor (AIF) and hypoxia-inducible factor (HIF-1?) in placentas of women with COVID-19 and critical lung lesion in a comparative aspect. Material and methods. The expression level of AIF and HIF-1? in placentas of women with COVID-19 was studied. The 1-st group consisted of placentas of 25 women
with COVID-19 and critical lung lesion (CLL) (more than 75 %, CT-4) after preterm labor induced by the severity of the mother's condition, and in every third case in combination with fetal distress; the 2-nd group consisted of placentas of 25 patients with COVID-19 and lung lesion of 25 – 50% (CT-2) after spontaneous preterm labor at the stage of clinical recovery after COVID-19. Results. The median area of immunopositive structures with respect to AIF was statistically significantly higher in placentas of group 2 and amounted to 66.38 (60.79; 74.51) % vs. 56.11 (28.04; 63.18) % (p = 0.000). The median area of immunopositive structures against HIF-1? was 17.80 (13.33; 27.33) % and 33.01 (27.63; 35.99) % respectively groups (p = 0.000). Conclusion. In patients with COVID-19 and CLL, AIF expression level was statistically significantly higher than the same parameter in placentas of women with COVID-19 and 25 – 50% lung lesion (CT-2) (p = 0.000), which may indicate the energy deficit of cyto- and syncytiotrophoblast cells due to the severity of the condition of women with CLL. On the contrary, the level of HIF-1? expression in the placenta structures of group 1 women was statistically significantly lower than in the placentas of group 2 patients (p = 0.000), which may be due to the delivery of COVID-19 and CLL patients before the development of severe hypoxic damage in the placentas.

Ekaterina E. Voropaeva – Professor of the V. L. Kovalenko Department of Pathological Anatomy and Forensic Medicine, South Ural State Medical University, Doctor of Medical Sciences, Professor. E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Yulia V. Hajdukova – postgraduate student of the Department of Obstetrics and Gynecology, South Ural State Medical University. E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Anna Yu. Holopova – postgraduate student of the V.L. Kovalenko Department of Pathological Anatomy and Forensic Medicine, South Ural State Medical University. E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Alina A. Alieva – postgraduate student of the V.L. Kovalenko Department of Pathological Anatomy and Forensic Medicine, South Ural State Medical University. E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Ella A. Kazachkova – Professor of the Department of Obstetrics and Gynecology South Ural State Medical University, Doctor of Medical Sciences, Professor. E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Evgeny L. Kazachkov – Head of the Department of Pathological Anatomy and Forensic Medicine named after Professor V.L. Kovalenko of South Ural State Medical University, Doctor of Medical Sciences, Professor E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

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DOI: 10.14489/lcmp.2024.02.pp.025-034
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Keywords

apoptosis, hypoxia, placenta, immunohistochemical study, COVID-19, critical lung lesion.

Use the reference below to cite the publication

Voropaeva EE, Hajdukova YuV, Holopova1 AYu, Alieva1 AA, Kazachkova EA, Kazachkov EL. Comparative analysis of apoptosis and hypoxia in placentas of patients with COVID-19 and critical lung lesion (immunohistochemical examination). Laboratory and Clinical Medicine. Pharmacy. 2024;4(2):25-34. (In Russ). DOI: 10.14489/lcmp.2024.02.pp.025-034